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. 2012 Apr 3;7(4):e34355. doi: 10.1371/journal.pone.0034355

Figure 4. EMSA experiments to assess binding ability and specificity of probes.

Figure 4

Panel (A): Shows the EMSA experiments to evaluate the specificity of the Oligo – Nuclear protein complex formed with the C and T probes. The Oligo–Nuclear protein complex was formed in the presence of 100× excess molar concentration of the unlabelled competitors namely Self / Heterologous / AP-4 consensus / Non specific (CEBP-α, NS) oligos. Panel (B): Bar diagram showing the densitometric profile of the autoradiograph, Figure 5.7, Panel A. The C & T type of oligo without nuclear lysate (Lanes 1 & 7) ; with nuclear lysate and without competitors (Lanes 2 & 8); with self competitors (Lanes 3 & 9) ; with heterologous competitors (Lanes 4 & 10 ); with unlabelled AP-4 consensus oligo ( Lanes 5 & 11) and with unlabelled non specific oligo (NS, CEBP-α, Lanes 6 & 12). The bars represent the mean ± SD of three experiments.