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. 2012 Apr 3;7(4):e34355. doi: 10.1371/journal.pone.0034355

Figure 5. Supershift assay using AP-4 consensus, wild type and polymorphic type probe incubated with / without AP-4 polyclonal antibody.

Figure 5

The assay carried out utilizing different probes without nuclear lysate ; (lanes 1 , 4 & 7); with Jurkat nuclear lysate ; (Lanes 2,5 & 8) Super shift assay with Jurkat cell nuclear extracts was carried out using anti AP-4 antibody (20 µl ) with specific AP- 4 probe (lane 3), C and T type probes, lanes 6 & 9 respectively. The arrow head indicates the shifted band corresponding to the different type of probes. The star symbol indicates supershifted complex corresponding to different probes. These data are representative of 2 independent experiments.