Table 2. List of primers used in the study.

Assay	Name	Sequence
Sequencing	IL-12F-780	5′ - agagcggttttaaggtaatgccca - 3′ (622 bp )a
	IL-12R -159	5′ - actcccgtataggtcccgtgtt - 3′
Cloning	−591Fb	5′- gcgcgagctcgatatctaaataaaatctct - 3′ (646 bp)
	+55Rc	5′- agttccctgatggctgtcaaca- 3′
Mutagenesis	FTMU	5′- attatgcagagttgccgacccct - 3′
	RTMU	5′- aggggtcggcaactctgcataat - 3′
EMSA	C probe S	5′- attatgcagagctgccgacccctct - 3′
	C probe AS	5′- agaggggtcggcagctctgcataa t-3′
	T probe S	5′- attatgcagagttgccgacccctct - 3′
	T probe AS	5′- agaggggtcggcaactctgcataat -3′
	AP4-S	5′- cacccggtcagctggccctacacc -3′
	AP4- AS	5′- ggtgtagggccagctgaccgggtg -3′
	C/EBP-α S	5′- atgtttttatgtaataaaa -3′
	C/EBP-α AS	5′- ttttattacataaaaacat -3 ′
Real time Primers	β-Actin –F	5′- agaggggtcggcaactctgcataat -3′ (101 bp )
	β-Actin –R	5′- atgctatcacctcccctgtgtg - 3′
	IL-12mRNA-F	5′- cctgtatcaatagtgatgaaattc -3′ (103 bp )
	IL-12mRNA-R	5′- tcccttctgtatgcaggataaat -3′
	IL-4RT-F	5′- aacagcctcacagagcagaagac - 3′ (101 bp )
	IL-4RT-R	5′- gccctgcagaaggtttcctt - 3′
	GATA3RT-F	5′- gcgggctctatcacaaaatga -3′ ( 79 bp )
	GATA3RT-R	5′- gctctcctggctgcagac-3′
	IFNαRT- F	5′- gctgaatgacctggaagcctgtg - 3′ (169 bp )
	IFNαRT-R	5′- gatttctgctctgacaacctccc - 3′

^aAmplicon size.

^b SacI site in bold incorporated in the −591F primer.

^c NheI site in bold incorporated in the +55R primer.