Skip to main content
. 2012 May 1;139(9):1547–1556. doi: 10.1242/dev.077412

Fig. 3.

Fig. 3.

Ago1 and Mei-P26 regulate orb translation in GSCs. (A) mei-P26mfs1 clonal germarium stained for GFP (green), Orb (red) and DNA (blue). The negatively marked mei-P26 mutant GSC (arrow) expresses higher levels of Orb protein than its heterozygous neighbor (arrowhead). (B) Ago114 clonal germarium stained for GFP (green), Orb (red) and DNA (blue). The negatively marked Ago1 mutant GSC (arrow) expresses higher levels of Orb protein than its heterozygous neighbor (arrowhead). (C) A germarium expressing an Hsp83-lacZ-orb3′UTR reporter stained for lacZ (green), Orb (red) and DNA (blue). (D) RT-PCR of orb and Actin 5C mRNA from Ago1 and Mei-P26 IPs. orb mRNA was consistently enriched in the Ago1 and Mei-P26 IP pellets but not in the IgG control pellets. (E) The ratio of mRNA pulled down in a Mei-P26 IP versus a control IP and in an Ago1 IP versus a control IP as measured by real-time PCR. Error bars indicate s.d. Scale bars: 10 μm.