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. 2012 May 1;139(9):1577–1586. doi: 10.1242/dev.076851

Fig. 1.

Fig. 1.

Teratoma-susceptibility germ cells delayed entry into G0 arrest. Male germ cell proliferation at E15.5 associates with increased teratoma risk. (A) Oct4::GFP transgenic FVB (teratoma-resistant), 129 (low teratoma risk) and M19 (high teratoma risk) embryonic testes were sectioned and immunostained for the nuclear mitotic marker KI67 (E13.5 and E14.5) or KI67 and the EC cell marker E-cadherin to exclude EC cells from the germ cell counts (E15.5 and E16.5). Data are plotted as the percentage of KI67-positive germ cells ± s.e.m. (n=6-8). Means without a common letter differ, P<0.05. (B) Confocal microscopy images of an E16.5 M19 testis immunostained for KI67 and E-cadherin. A proliferating cluster of cells with an EC cell-like morphology, positive for E-cadherin and expressing low levels of the Oct4::GFP transgene is noted (arrows). E-cadherin-negative, Oct4::GFP-positive germ cells are not expressing KI67. Scale bar: 75 μm.