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. 2012 May 1;139(9):1577–1586. doi: 10.1242/dev.076851

Fig. 3.

Fig. 3.

Teratoma-susceptible germ cells retain pluripotency. NANOG expression at E15.5 associates with increased teratoma risk. (A) Oct4::GFP transgenic FVB, 129 and M19 embryonic testes were sectioned and immunostained for NANOG (E13.5 and E14.5) or NANOG and E-cadherin (E15.5 and E16.5). Data are plotted as the percentage of NANOG-positive germ cells ± s.e.m. (n=6-8). Means without a common letter differ, P<0.05. (B) Confocal microscopy images of E13.5-E15.5 testis immunostained for NANOG. At E15.5 FVB germ cells are NANOG-negative, most 129 germ cells are NANOG-negative, and many M19 germ cells are NANOG-positive. Small groups of NANOG-positive germ cells are noted (arrowheads). Scale bar: 75 μm.