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. 2012 May 1;139(9):1662–1669. doi: 10.1242/dev.075051

Fig. 6.

Fig. 6.

The altered PVC does not result from extensive cell loss in Wnt1 morphants. (A) PVC progeny are present in the ectoderm between the blastoporal and stomodeal regions in 3-day old Wnt1 morphants. The posterior half of the embryo was labeled red by photoactivating KikGR at early mesenchyme blastula stage (control embryo, Aa-c; Wnt1 morphant, Aa′-c′) and photographed at 3 days post-fertilization to monitor the positions of red cells in the PVC (same control embryo, Ad-f; same Wnt1 morphant, Ad′-f′). The yellow arrows in Af′ indicate the PVC progeny between the blastopore and stomodeal regions. (B) No caspase 3 (green) is detected in Wnt1 morphants. (Ba,a′) Control 3-day embryo; (Bb,b′) cadmium chloride-treated 3-day embryo; (Bc-e,c′-e′) Wnt1 morphants at the indicated times. Asterisks mark mouth in (Ad,Ba) and stomodeal ectoderm in (Ad′). White arrows indicate blastopore in (Ad′,Bc). Scale bar: 20 μm.