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. 2012 Apr 3;7(4):e34376. doi: 10.1371/journal.pone.0034376

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Tundo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Zymographyc analysis of cell culture supernatants harvested after 24 hrs of incubation indicate that somatostain administration does not modulate gelatinolytic activity of MMP-9 in each of the experimental conditions. (B) In Western blotting analysis of cell lysates a polyclonal anti-MMP-9 antibody recognizes a single band corresponding to the pro-MMP-9 (92 kDa) which is not modulated over each experimental condition. Analysis of β-tubulin represents the internal control. The results presented are the means ± ES of three indipendent experiments in triplicate, n = 9.