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. 2012 Apr 3;7(4):e34563. doi: 10.1371/journal.pone.0034563

Figure 2. Affinities of wild-type 933W repressor protein for synthetic 933W operators.

Figure 2

The affinities of 933W repressor for the substituted DNAs are expressed as the dissociation constants of these operators for 933W repressor. NS-DNA that does not contain a DNA sequence to which 933W repressor binds specifically. The affinity of 933W repressor for non-specific DNA is give as an intrinsic ‘per-site’ dissociation constant (KD NS = KD OBS[2L-n], where L is DNA length and n is site size [28]). The protein concentrations used were corrected for activity.