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. 2012 Apr 3;7(4):e34860. doi: 10.1371/journal.pone.0034860

Figure 2. Chronic hyperglycemia alters the responsiveness to cAMP in pancreatic islets.

Figure 2

(A) Rat islet cells were cultured in the presence of 5 mM or 30 mM glucose for 8 days and stimulated with 30 µM forskolin for 0–12 h after 2 h preconditioning in 5 mM glucose. (B∼F) Real-time PCR was carried out using SYBR green to quantitate the mRNA levels of indicated genes in diverse conditions shown in A. Relative mRNA levels were estimated from Ct values summarized in Supporting Table S1 using 2−ΔΔCt method. The data from three independent experiments are presented as average fold ratios of relative mRNA expression compare with the 5 mM glucose-cultured islets before forskolin treatment in 5 mM glucose-cultured islets. Significant effects of forskolin (*, P<0.05; **, P<0.01) or 8-day incubation in 30 mM glucose (#, P<0.05) are marked.