Figure 1. Gating of VSEL cells and CD34+CD45dim cells in hUCB.

hUCB TNC were isolated by NH₄Cl lysis and stained with fluorescent antibodies, Hoechst 33342, and PI. A) Gating of living (PI⁻) nucleated (Hoechst 33342⁺) cells; B) pulse processing: exclusion of doublets (Hoechst-W^high) and granulocytes (SSC-A^high). C) Gating of CD34⁺CD45^dim Hoechst 33342⁺PI⁻. D) Gating of CD45⁻Lin⁻ cells. E) Gating of CD45⁻Lin⁻ CXCR4⁺Hoechst 33342⁺PI⁻ VSEL cells. F) Expression of CD133 in CD34⁺CD45^dim cells (blue) and VSEL cells (black).