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. 2012 Apr 3;7(4):e34899. doi: 10.1371/journal.pone.0034899

Figure 2. Immunophenotype of CD45-Lin-Hoechst 33342+PI- cells.

Figure 2

hUCB cells were subjected to multiparameter FACS analysis, and gated according to Figure 1 D (either Lin− or CD235a−). Among 22 markers tested, three were found positive on CD45−Lin− cells: CXCR4, CD31 and CD84. However, in contrast to previous reports, CD45−Lin− cells contain very low amounts of either CD34+ (1,2%+/−0,6%; n = 5) or CD133+ (1,8%+/−2,3%; n = 5) cells (B middle & right). A) Representative single analysis of markers on CD45−Lin− cells. B+C) Representative replicate analysis of the expression of CXCR4, CD34 and CD133 on CD45−Lin− cells and on CD34+CD45dimcells.