Figure 1.

Functions of the average particle size and ζ-potential on pH for the aqueous suspensions of rH174 (a), rH163 (b), and rH146 (c) (N = 15, 6, 4, respectively), including the amino acid sequences thereof (d). The graphs were made by overlapping the results of analyses of proteins derived from different recombinant batches, and each datapoint was obtained as an average of over 100 acquisitions. The bolded amino acids in the sequences of rH174, rH163, and rH146 represent the residues with titratable side-chains.