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. 2010 Feb;89(2):186–191. doi: 10.1177/0022034509354843

Figure 2.

Figure 2.

Activations of NOD1 and NOD2 by sPGNs from periodontopathic bacteria. (A) Diptericin expression in Drosophila S2* cells by stimulation with purified sPGNs from periodontopathic bacteria. S2* cells stably transfected with diptericin-luciferase reporter plasmid were seeded into 96-well plates and differentiated into macrophage-like cells by incubation with 1 µM 20-hydroxyecdysone for 24 hrs. Then the cells were stimulated with sPGN samples, and the luciferase activities were measured 4 hrs after stimulation and expressed as means ± SD of triplicate experiments. The results are representative of 3 different experiments. HEK293T cells were transfected for 24 hrs with pUNO/hNod1 (B) or pUNO/hNod2 plasmid (C) together with NF-κB-firefly luciferase and renilla luciferase reporter plasmid. After 24 hrs, the cells were stimulated with sPGN samples. Then luciferase activities were measured 12 hrs after stimulation and expressed as means ± SD of triplicate experiments. The results are representative of 3 different experiments. P.g., P. gingivalis ATCC 33277; A.a., A. actinomycetemcomitans; F.n., F. nucleatum; E.c., E. coli; A.v., A. viridans. *P < 0.05, **P < 0.01 vs. (-); #P < 0.05, ##P < 0.01 vs. P.g.