Table 1.
Embryonic Day 15 Bud vs. Main Duct, Selected Functions, and Pathways of Genes Differentially Expressed
| Gene Symbol | Fold Change in Microarray | Fold Change by qPCR | References with Localization Data |
|---|---|---|---|
| Adherens junction/Tight junction/Cell adhesion/Focal Adhesion | |||
| Cdh1 | −1.5 | −4.6 | – |
| Cldn10 | a | – | IF: E17 bud; Appendix Ref.1 |
| Cldn11 | b | – | IF: E16 duct; Appendix Ref.1 |
| Cldn4 | −7.4 | – | IF: E16 duct; Appendix Ref.1 |
| Egfr | −4.3 | c | IF: ducts; Appendix Ref.2 |
| Cldn6 | b | – | IF: E16 duct; Appendix Ref.1 |
| Itga6 | 1.2 | 1.5 | IF: epithelium; Appendix Ref.3 |
| Krt17 | −3.7 | −11 | – |
| Krt18 | −1.8 | −3.5 | IF: epithelium; Appendix Ref. 4 |
| Krt19 | −5 | – | ISH: ducts; Appendix Ref. 5 |
| Krt7 | −17 | −66 | ISH: ducts; Appendix Ref. 5 |
| Krt8 | −1.4 | −1.7 | IF: epithelium; Appendix Ref. 4 |
| Lamb3 | −16.9 | −16.7 | – |
| Substrate Specific Channel Activity | |||
| Kcnn4 | 49 | – | IF: late bud; Appendix Ref. 6, 7, 8 |
| Aqp5 | 28 | – | qPCR: late bud; Appendix Ref. 9, 10 |
| Cftr | −9.2 | – | IF: ducts; Appendix Ref. 11, 12 |
| Scnn1a | −20.5 | c | – |
| Scnn1b | −25 | – | ISH: E16 duct; Appendix Ref. 5 |
| Wnt signaling pathway | |||
| Wnt10a | −155 | −941 | – |
| Wnt6 | −2.5 | −56.4 | – |
| Ctnnb1# | −2.3 | 0 | – |
| Non-categorized genes | |||
| Dcpp1 | 84 | 9 | ISH: E14.5; Appendix Ref. 13, 14 |
| Dcpp2 | 92 | 20 | ISH: E14.5; Appendix Ref. 13, 14 |
| Dcpp3 | 52 | 8 | ISH: E14.5; Appendix Ref. 13, 14 |
| Enzyme activator activity | |||
| Acap3 | 5.3 | – | – |
| Gpsm3 | 20.5 | – | – |
| Sec14l2 | 10.6 | – | – |
| Mgst2 | 6.5 | – | – |
| Rap1gap | 6.6 | – | – |
| Transcription factor activity | |||
| Runx2 | −6.1 | – | – |
| Jun | −7.8 | – | – |
| Creb5 | −31.2 | – | – |
| Dach1 | −11.5 | – | – |
| Mafb | −15.3 | – | – |
| Meis2 | −15 | – | – |
Key: −, no expression data at either the appropriate age or location available for that gene; #, qPCR data disagree with microarray results; a - no expression detected in duct arrays; b - no expression detected in bud arrays; c - not detected in bud samples; d - no expression detected in E12.5 bud; qPCR - localization by qPCR; IF - localization by immunofluorescence; ISH - localization by in situ hybridization.