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The ninhydrin test shows that dissolved amounts of free amino acid after 50 mM EDC/NHS and 80 mM EDC/NHS treatment were decreased. Suberic acid, procyanidin, DPPA and butanal-Jaffmine group showed weak nature to the ninhydrin test similar to the pronase test
GA: glutaraldehyde, De: decellurarization, EDC: ethyl-dimethylaminopropyl carbodiimide hydrochloride, NHS: N-hydroxysucinimide, DPPA: diphenylphosphoryl azide, DIA: hexane-1-6 diamine, SUA: suberic acid
