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. 2001 Apr 10;98(9):5164–5169. doi: 10.1073/pnas.091537598

Figure 1.

Figure 1

Selective developmental defect in NK cells of the Id2−/− fetal thymus. (A) Cells of 17-dpc FT were stained with CD3-, NK1.1-, CD4-, and CD8-specific mAbs. Total thymocyte numbers were 1.5 ± 0.9 × 106, 1.8 ± 0.6 × 106 and 0.7 ± 0.6 × 106 for Id2+/+, Id2+/−, and Id2−/− FT, respectively. (B) Cells of 14-dpc FT were stained with FITC-conjugated mAbs specific for TER119, Mac-1, Gr-1, B220, NK1.1, CD3, CD4, and CD8. Only FL-1-negative cells were analyzed for expression of CD44 vs. CD122 and CD44 vs. CD25. Total thymocyte numbers were 5.6 ± 1.4 × 104, 3.2 ± 1.4 × 104, and 1.5 ± 1.3 × 104 for Id2+/+, Id2+/−, and Id2−/− FT, respectively. Numbers represent the percentage of cells in the gated population and in each quadrant in A and B.