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. 2001 Apr 10;98(9):5164–5169. doi: 10.1073/pnas.091537598

Figure 4.

Figure 4

Reverse transcription–PCR analysis for the gene expression of Id and E proteins in subpopulations of fetal thymus. Wild-type thymocytes at 15 dpc were fractionated, based on cell surface phenotype, into developmental subsets. Lin− TN CD122+ cells in FT are exclusively NK lineage committed, as previously reported (10). mRNA was prepared from sorted cells (as indicated at the Top of the figures) and reverse-transcribed. The cDNA was amplified with specific primers for each gene indicated. Reverse transcription–PCR and PCR products of 14-dpc embryonic head total RNA served as positive and negative controls, respectively, in each reaction.