Figure 3.

MK 8745 induces apoptosis in a p53 dependent manner. (A) Cell lines different lineages; (i) colon (HCT116 and its p53 null variant), (ii) pancreatic (PANC1, p53 mutant and CAPAN2, p53 wild type), (iii) sarcoma (ST88, p53 mutant and DSCRT, p53 wild type) and (iv) melanoma (SKMel 28, p53 mutant and SKMel 32, p53 wilt type) were treated with MK8745 (5 µM) for 48 h and harvested for flow cytometry for the determination of cell cycle distribution. (B) All the cell lines in (A) were treated with MK8745 and harvested for western blot analysis for induction of PARP cleavage. Results shown are representative of at least three independent experiments.