Figure 6.

p53 is critical in determining the fate of the cell when Aurora A is inhibited. (A) (i) HCT116 cells and p53^−/− cells either transfected with vector or with p53 were treated (MK, 5 µM for 24 h) western blot analysis was performed for PARP cleavage and expression of p53. (ii) Apoptosis was quantitated by microscopy after staining with DAPI (gray, DMSO and black, MK). (B) Polyploidy and apoptosis were quantitated by FACScan analysis after staining with PI in all conditions mentioned in the previous experiment at 0, 6, 18, 24, 30, 44 and 52 h. (C) p53^−/− cells overexpressing p53 were treated with MK (5 µM) for 24 h and immunofluorescence assay was performed for p53 (green) followed by DAPI stain (blue). All the results shown are representative of 3–4 independent experiments.