Figure 3.
Spreading in the presence of serum is qualitatively different. (a) Time-lapse IRM images of a T cell spreading on antibody-coated glass substrate in the presence of serum. Scale bar, 5 μm. (b) Contact area of the cell as a function of time. Each graph corresponds to a different cell. The solid gray lines show a fit to a hyperbolic tangent function. (c) Comparison of spreading rate, α, and final spread area, A0, for serum and serum-free cases shows that these parameters are very similar in the two conditions. (d) Kymographs of four representative sections in serum. (e) Kymographs of two representative sections in serum-free conditions (scale bars, 3 μm, 30 s).