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. 2012 Apr 4;102(7):1524–1533. doi: 10.1016/j.bpj.2012.02.015

Figure 6.

Figure 6

Dynamics and spatial distribution of ZAP70 clusters is distinct in the two modes of spreading. (a) Time-lapse TIRF image of T cells expressing YFP-ZAP70 spreading on an antibody-coated glass substrate in serum-free media. The cyan (inner) line represents the boundary of the clustered zone at any given time point. The red (outer) line denotes the final boundary of the fully spread cell. (b) Time-lapse TIRF image of T cells spreading in serum. To facilitate comparison, the three time-lapse images shown are at 40 s, 80 s, and 160 s (corresponding to rescaled times of 1, 2, and 4). (c) TIRF image of a typical YFP-ZAP70 cell in serum-free media. (d) TIRF image of a typical YFP-ZAP70 cell in serum containing media. Scale bars, 5 μm. (e) Intensity-weighted second spatial moment of fluorescent clusters show that ZAP70 clusters are more peripheral in serum-free conditions but uniformly distributed in serum (p < 0.001, Wilcoxon rank test).