Figure 1.

Primary sequence analysis of PQBP-1. (a) DISOPRED2 prediction of local disorder as a function of residue number. The dotted line indicates a 5% false-positive threshold. The domain structure of PQBP-1 is shown at the bottom. (b) Plot of net charge versus hydropathy as a predictor of disorder, compared with a database of folded (gray squares) and unfolded (open circles) proteins. The position of PQBP-1 is shown as a black circle. The boundary condition is shown as a sloping line having the equation $\langle R\rangle =2.785\langle H\rangle -1.151$, where $\langle R\rangle$ is the absolute mean net charge and $\langle H\rangle$ is the mean scaled hydropathy (20).