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. 2012 Apr 4;102(7):1493–1502. doi: 10.1016/j.bpj.2012.03.005

Figure 4.

Figure 4

p21-EGFP/LifeAct-mCherry measurements indicate that Arp2/3 complex accumulation precedes F-actin accumulation. (A) XTC cell expressing p21-EGFP and LifeAct-mCherry. (Right panels) Montage of a indicated section of the leading edge over time (frames are 10 s apart). p21-EGFP accumulates at the leading edge during protrusion and dissipates during retraction, preceding F-actin. Retrograde flow remains unchanged, 48 ± 1 nm/s (n = 15 measurements of bright features). (B) Total p21-EGFP intensity versus angle and time, over a 5-μm-wide band of lamellipodium. (C) Same as panel B, for LifeAct-mCherry. Protracted horizontal patterns are due to filopodia. (D) Average correlation coefficients versus time and with no positional offset for p21-EGFP autocorrelation, LifeAct-mCherry autocorrelation, and cross correlation of p21-EGFP with LifeAct-mCherry. (E) Same as panel D, but as a function of arc length and no time offset. (F) Average intensity of p21-EGFP and LifeAct-mCherry versus distance from the leading edge.