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Cell cycle analysis. HeLaPKCεA/E cells were incubated in the presence or absence of 2 μg/ml doxycycline and/or 15 μM zapotin for 24 h. Cells were collected (using trypsin) and the cell pellet was resuspended in propidium iodide/ribonuclease and incubated for 1 h, followed by flow cytometry analysis. *, P < 0.05, compared to the controls.
