Table 1.
Direct Sanger sequencing of RT-PCR products amplified from each viral genome segment in egg-passaged viruses.
| Segment | #1 (P6) |
#2 (P6) |
#3 (P5) |
|||
|---|---|---|---|---|---|---|
| nt | aa | nt | aa | nt | aa | |
| PB2 | 0 | 0 | 0 | 0 | 0 | 0 |
| PB1* | 0 | 0 | 0 | 0 | 0 | 0 |
| PA | 0 | 0 | 0 | 0 | 0 | 0 |
| HA* | G427A | E66 K | G408T | K119N | G408T | K119N |
| A424G | N125D | A716G | D222G | A716G | D222G | |
| A719G | Q223R | A719G | Q223R | T1161A | N370K | |
| NP | T159G | D53E | 0 | 0 | G447A | D375N |
| G1123A | ||||||
| NA | 0 | 0 | 0 | 0 | 0 | 0 |
| M | 0 | 0 | 0 | 0 | 0 | 0 |
| NS | 0 | 0 | 0 | 0 | 0 | 0 |
P6 generation was studied for #1 and #2-derived viruses and the P5 generation was studied for the #3-derived viruses.
The results show nucleotide (nt) and amino acid (aa) substitutions compared with A/Osaka/01/2009.
*R634H found within PB1 and I142V found within HA were commonly detected in all egg-adapted viruses as well as the viruses in the clinical specimen (#2).