Table 3.

Frequency of multiple mutations in nine amino acids of the H1N1pdm HA in egg-passaged (P1 and P6), and MDCK-passaged samples derived from #2.

		119	125	129	133	183	187	215	222	223	Total (%)
Egg (P1)	Wild type	K	N	N	T	S	D	A	D	Q	2.94
	Mutation	N									0.29
			D								0.59
								V			2.35
									G		13.8
										R	65.6
		N								R	0.29
			D						G		9.41
			D							R	2.06
				S						R	0.29
						P				R	0.59
							E			R	0.29
								V		R	0.29
									G	R	1.18
Egg (P6)	Wild type	K	N	N	T	S	D	A	D	Q	0.5
	Mutation	N									1.98
			D								0.5
									G		0.5
										R	0.5
		N		S							3.96
		N							G		6.93
			D				E				0.5
			D						G		0.99
			D							R	7.92
				S					G		3.96
				S						R	0.5
		N		S				V			0.5
		N		S					G		45.5
			D				E		G		0.5
			D				E			R	21.8
		N		S			E		G		0.5
		N		S			E			R	0.99
			D				E		G	R	1.48
MDCK	Wild type	K	N	N	T	S	D	A	D	Q	98.84
	Mutation				S						0.19
						P					0.19
								V			0.39
									G		0.39

Critical substitutions such as K119N, N125D, N129S, D187E, A215V, D222G, Q223R, and two additional substitutions T133S and S183P, as controls, are shown.