Table 1.

Pathophysiology study design

Group and treatment	No. of mice	Sample collection, h postchallenge
		12	24	36	48	60	72
Aerosol exposurea
1	20	X
2	20		X
3	20			X
4	20				X
5	20					X
6	20						X
Baseline controlb
7	10	X
Media controlsc
8	12	X					X

^aMice were challenged with a lethal dose of Y. pestis CO92 via nose-only exposure. At each time point, terminal blood samples were collected and pooled by sex for clinical chemistry and bacteremia determinations. Differential white blood cell counts and hematocrit were performed on individual samples. Gross necropsies, bacterial tissue burdens, and histopathology were completed at each time point.

^bUntreated mice served as baseline controls for clinical chemistry and hematology.

^cAerosol exposure of control material consisting of fresh, sterile growth medium used to propagate Y. pestis CO92. Six mice (three males and three females) were euthanized at 12 and 72 h and served as pathology controls.