Fig 5.

Flow cytometry analysis of inhibition of FH binding to D39 through anti-PspC IgG. Binding of IgG (A and B), binding of FH (C and D), and blockage of binding of FH by anti-PspC IgG (E and F) were analyzed by flow cytometry. The pneumococcal strains D39 (A, C, and E) and D39-ΔpspA (B, D, and F) were employed in the assays. Bacteria incubated only with the detection antibody conjugated with FITC were used as a control for each strain. Samples without incubation with FH (C and D) and samples incubated with anti-intimin IgG (E and F) are also shown.