Fig 8.

The M1 carrier enhances anti-OVA Ab responses in wild-type and transgenic mice. C57BL/6 (a), humanized DR3-DQ2 (b), or humanized DR4-DQ8 (c) transgenic mice were given a single subcutaneous injection with 10 μg OVA alone or 10 μg OVA coupled to M1 emulsified in IFA. The development of a circulating Ab response to OVA was determined by ELISA at 2, 3, 5, and 8 weeks postimmunization. (d) The ratio of IgG1 Ab/IgG2c Ab was measured by ELISA on week 8. All ELISA data are displayed as whisker plots showing the minimum to maximum range of data points, and the horizontal line represents the median value. Data shown contain 5 mice/treatment group and 30 mice in total and are representative of two independent experiments. P values were calculated using a one-tailed Mann-Whitney test.