Fig 1.
Immunoblotting to detect S. aureus-specific antibody responses. Lysate (5 μg/lane) from heat-killed S. aureus was loaded and probed with preimmune (lane 1) and postimmune (lane 2) bovine serum obtained after the fourth immunization and diluted 1:100 or 1:1,000. Alkaline phosphatase-conjugated mouse anti-bovine IgG (1:5,000) was used as a secondary antibody, and blots were developed in a substrate solution containing NBT/BCIP for 1 to 5 min. Asterisks indicate unique bands which appeared after the fourth immunization. Mr, relative molecular weight in thousands.