Fig 3.

Antigens that induced significant proliferation for one or more calves. Proliferation assays were performed with 2-week T cell lines from the immunized calves using 1 and 5 μg/ml protein-G bead-bound IVTT-expressed S. aureus proteins. Negative-control antigens include IVTT-expressed A. marginale VirB9-2 beads and no-DNA control beads. Data are presented from one screening assay for each animal. Responses were considered significant (indicated by asterisks) if mean the cpm was >1,000, the SI compared with negative-control antigens was >2.0, and P was <0.05 compared to negative-control antigens and medium (using ANOVA with Dunnett's posttest for multiple comparisons).