Fig 4.

S. aureus antigens do not induce significant proliferation of 2-week T cell lines from A. marginale-immunized cattle. S. aureus proteins were tested on 2-week T cell lines from cattle immunized with A. marginale OM: animal 35287 that expresses the DRB3 RFLP haplotype 16/22 (A) and animal 33583 that expresses the DRB3 RFLP haplotype 8/23 (B). A. marginale OM and IVTT-expressed VirB9-1 (CTP) and VirB9-2 (VirBa) bound to protein G beads were used as positive controls. Responses were considered significant (indicated by asterisks) if the mean cpm was >1,000, the SI compared with negative-control antigen was >2.0, and P was <0.05 compared to negative-control antigens and medium (using ANOVA with Dunnett's posttest for multiple comparisons).