Table 2.
Summary of antigens that induced significant T cell proliferation in three experiments using 2-week cell lines from S. aureus-immunized calves
| Expt no. | Proliferative response of T cell lines to the indicated antigen(s) by animala |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 30864 (12/16)b |
30900 (23/27) |
30904 (11/16) |
30916 (3/24) |
30919 (10/22) |
||||||
| Antigen | SI | Antigen | SI | Antigen | SI | Antigen | SI | Antigen | SI | |
| 1 | NWMN_0364 | 25.7c | NWMN_2086 | 9.6c | NWNM_0601 | 38.2c | NWMN_0601 | 2.3c | NWMN_2086 | 8.3c |
| NWMN_0601 | 24.4c | NWMN_0364 | 2.4 | NWMN_1999 | 4.3 | |||||
| 2 | NWMN_0364 | 46.7c | NWMN_2086 | 17.2c | NWMN_0601 | 6.7c | NWMN_0601 | 5.3c | NWMN_2086 | 9.3c |
| NWMN_0601 | 20.1c | NWMN_0601 | 3.0d | NWMN_0429 | 4.4 | NWMN_2086 | 2.2d | NWMN_2469 | 2.6d | |
| NWMN_0161 | 25.3 | NWMN_2270 | 2.3d | |||||||
| NWMN_0390 | 10.4 | NWMN_2318 | 7.8d | |||||||
| NWMN_2319 | 7.2d | |||||||||
| NWMN_2320 | 2.2d | |||||||||
| NWMN_2469 | 9.6d | |||||||||
| 3 | NWMN_0364 | 38.9c | NWNM_2086 | 4.2c | NWMN_0601 | 2.7c | NWMN_0601 | 18.9c | NWMN_2086 | 6.0c |
| NWMN_0601 | 19.3c | NWMN_0601 | 2.4d | NWMN_1733 | 2.7 | NWMN_2086 | 12.5d | NWMN_2469 | 13.7d | |
| NWMN_2086 | 9.4 | NWNM_2320 | 4.7 | NWMN_2270 | 12.8d | NWMN_2320 | 5.3 | |||
| NWMN_2318 | 29.3d | |||||||||
| NWMN_2319 | 24.0d | |||||||||
| NWMN_2320 | 16.2d | |||||||||
| NWMN_2469 | 34.5d | |||||||||
Different batches of IVTT-expressed proteins were tested in each experiment. Antigens were determined to induce significant responses based on the following criteria: mean cpm of >1,000, SI of >2.0, and P of <0.05 compared to medium, beads, and VirB9 beads. Significant proliferation was determined using ANOVA with Dunnett's posttest for multiple comparisons (P < 0.05). Assays were performed using 43 antigens tested in triplicate from three expression experiments. SI was determined as the mean cpm in response to 1 or 5 μg/ml S. aureus antigen/mean cpm in response to Virb9 beads, whichever was higher.
MHC class II haplotypes were determined by RFLP analysis of exon II from the DRB3 locus. For example, 12/16 refers to the RFLP haplotypes designated DRB3 12 and DRB3 16, each inherited from one parent.
Antigens that induced significant proliferation in three independent assays for each animal.
Antigens that induced significant proliferation in two independent assays for each animal.