Table 3.
IFN-γ induced by S. aureus proteins
| Culture condition | Amt of antigen (μg/ml) | IFN-γ secretion (U/ml) by 2-week cell lines from the indicated animala |
||||
|---|---|---|---|---|---|---|
| 30864 | 30900 | 30904 | 30916 | 30919 | ||
| Medium | 1.0 | 40.0 | 1.0 | 1.0 | 6.4 | |
| S. aureus cells | 429.6 | 298.0 | 596.2 | 111.5 | 294.3 | |
| TCGF | 415.1 | 235.0 | 171.1 | 126.1 | 239.8 | |
| IVTT-expressed protein | ||||||
| VirB9 | 1 | 2.0 | 33.0 | 7.0 | 12.3 | 190.4 |
| 5 | 8.1 | 60.0 | 24.0 | 21.8 | 251.2 | |
| NWNM_0161 | 1 | 16.1 | ND | 126.5 | ND | ND |
| 5 | 28.5 | ND | 29.8 | ND | ND | |
| NWMN_0364 | 1 | 325.5* | ND | ND | ND | 225.0 |
| 5 | 401.1* | ND | ND | ND | 219.4 | |
| NWMN_0390 | 1 | 22.5 | ND | ND | ND | ND |
| 5 | 30.7 | ND | ND | ND | ||
| NWMN_0429 | 1 | 29.5 | 33.4 | 0.6 | ND | ND |
| 5 | 16.1 | 33.0 | 1.0 | ND | ND | |
| NWMN_0601 | 1 | 381.9* | 25.1 | 19.8 | 39.1 | ND |
| 5 | 429.2* | 43.2 | 11.3 | 90.3* | ND | |
| NWMN_0724 | 1 | 2.08 | ND | 34.6 | ND | ND |
| 5 | 17.4 | ND | 15.1 | ND | ND | |
| NWMN_1733 | 1 | 1 | ND | 114.5* | ND | 139.8 |
| 5 | 17.68 | ND | 80.0* | ND | 196.5 | |
| NWMN_1880 | 1 | ND | ND | ND | 12.4 | ND |
| 5 | ND | ND | ND | 24.6 | ND | |
| NWMN_1999 | 1 | ND | ND | ND | ND | 168.4 |
| 5 | ND | ND | ND | ND | 211.1 | |
| NWMN_2086 | 1 | 17.1 | 206.3* | ND | 13.4 | 179.8 |
| 5 | 49.7 | 198.0* | ND | 40.4 | 211.2 | |
| NWMN_2270 | 1 | 67.7* | 55.4 | ND | 11.7 | ND |
| 5 | 99.9 | 36.4 | ND | 25.6 | ND | |
| NWMN_2318 | 1 | ND | 66.4 | ND | 27.5 | 258.8 |
| 5 | ND | 87.4 | ND | 53.1* | 273.7 | |
| NWMN_2319 | 1 | 36.8 | 34.1 | ND | 26.3 | ND |
| 5 | 73.5* | 26.4* | ND | 44.4* | ND | |
| NWMN_2320 | 1 | 22.8 | 87.1 | ND | 10.9 | 220.1 |
| 5 | 45.3 | 108.2 | ND | 20.3 | 213.5 | |
| NWMN_2469 | 1 | 39.8 | ND | ND | 145.9* | 212.8 |
| 5 | 63.8 | ND | ND | 219.4* | 206.5 | |
| NWMN_2579 | 1 | 22.8 | 41.8 | ND | 11.4 | ND |
| 5 | 45.3 | 44.6 | ND | 60.7 | ND | |
a
Data are presented as the mean U/ml of IFN-γ secreted by 2-week T cell lines cultured in triplicate with 1 × 106 heat-killed S. aureus bacteria or the indicated antigens. Boldface numbers indicate significant (P < 0.05) IFN-γ secretion with respect to medium and bead-bound VirB9. Asterisks (*) indicate antigens that induced significant proliferation. ND, not determined.