Table 4.
Titers of IgG1 and IgG2 to recombinant proteins in cattle immunized with S. aureus
| Animal |
S. aureus-specific IgG titer to the indicated antigena |
|||||
|---|---|---|---|---|---|---|
| rNWMN_0364 |
rNWMN_0601 |
rNWMN_2086 |
||||
| IgG1 | IgG2 | IgG1 | IgG2 | IgG1 | IgG2 | |
| 30864 | 4,000 | 400 | 4,000 | 400 | 4,000 | 400 |
| 30900 | 4,000 | 400 | 4,000 | 400 | 4,000 | <400b |
| 30904 | 4,000 | 400 | 4,000 | 400 | 4,000 | <400 |
| 30916 | 4,000 | 400 | 4,000 | 400 | 4,000 | <400 |
| 30919 | 4,000 | 400 | 4,000 | 400 | 4,000 | <400 |
a
Serum obtained 2 weeks after the last immunization were adsorbed with E. coli lysate, diluted 1:400 to 1:4,000, and tested for reactivity to recombinant protein (indicated by the “r” prefix) by immunoblotting. The titer is defined as the reciprocal of the highest serum dilution giving a positive signal.
b
No specific bands were detected at a 1:400 dilution, and no other dilutions were tested.