Table 2.
List of MICA primer sequences, their positions, and the sizes of the amplified products
| PCR type and primer name | Primer sequence | Location (position [nt])a | Product size (bp) |
|---|---|---|---|
| MICA-specific PCR | |||
| MICA-FG | 5′-CGTTCTTGTCCCTTTGCCCGTGTGC-3′ | Intron 1 (6823–6847) | 2,201 |
| MICA-RF | 5′-GATGCTGCCCCCATTCCCTTCCCAAA-3′ | Intron 5 (8999–9023) | |
| MICA nested PCR | |||
| MICA-FM | 5′-GGGTCTGTGAGATCCATGA-3′ | Exon 3 (350–368) | 127 |
| MICA-RMb | 5′-TGAGCTCTGGAGGACTGGGGTA-3′ | Exon 3 (455–476) |
a
Positions are based on GenBank accession number X92841. nt, nucleotide.
b
The exon 3 reverse primer has a deliberately introduced mismatch to create an RsaI restriction site.