Fig 2.

Cin1, Cdc42, and Wsp1 function collectively to regulate the actin cytoskeleton. (A) F-actin was visualized using rhodamine-conjugated phalloidin staining of the indicated strains. All strains were incubated to the mid- and late-logarithmic phases in YPD prior to incubation in HBSS. WT, wild type. In the cin1 mutant background, the following alleles were expressed using the GPD promoter: the wild-type CDC42 gene, the dominant active CDC42^Q61L allele, the wild-type WSP1 gene, and the CDC42-WSP1 fusion allele. In the wsp1 background, the following genes/domains were overexpressed: the DH domain of the CIN1 gene and the PH domain of CIN1 coexpressed with the CDC42^Q61L allele. DIC, differential interference contrast. (B) GFP-Wsp1 and DsRed-Cdc42 were coexpressed in the wsp1 cdc42 mutant strain. An overlay of the two images suggests that Wsp1 and Cdc42 occupy shared subcellular domains in vivo.