Fig 2.

BUL gene deletions show complex growth phenotypes but are not required for ubiquitin-mediated processing of Mga2p. (A) Tenfold serial dilution of BUL gene deletion strains grown on YPD agar medium, YPD plus 25 μM CdCl₂ (Cd) or 3 μM phleomycin (Phl), or YEP plus 2% (vol/vol) ethanol (EtOH). (B) Tenfold serial dilution of BUL gene deletion strains grown on YPD agar medium plus 1 mM AZC, synthetic complete medium with no arginine (SC), or SC minus Arg plus 14 μM canavanine (Can). (C) Immunoblot using anti-HA antibodies of HA-Mga2p processing from P120 to P90 in wild-type and BUL gene deletion (Δ1,2,3) strains.