Figure 1.

Selective expression of gob-5 mRNA in airway epithelia from AHR-model mice. (A) Tissue distribution of gob-5 was determined by Northern blot analysis in normal and AHR-model mice. (Upper) The results obtained by hybridization with a gob-5 cDNA probe. The murine tissues from which the poly(A)⁺ RNA was prepared are indicated at the top. The same filter was rehybridized with a β-actin probe to control for equal loading (Lower). (B, C, E, and F) Lung sections of normal (B and C) and AHR-model mice (E and F) were in situ hybridized with DIG-labeled, single-stranded antisense (B and E) and sense (C and F) RNA probes. (D and G) The same lung sections of normal (D) and AHR-model mice (G) were stained with PAS and counterstained with hematoxylin. Original magnification, ×200. Three different experiments showed similar results.