Figure 4.

Mucus production in human mucoepidermoid cells transfected with gob-5 or hCLCA1. (A) PAS staining was performed on NCI-H292 cells after transfection with control vector (pcDNA3.1), gob-5 expression vector (pcDNA-gob-5), or hCLCA1 expression vector (pcDNA-hCLCA1). (B) Induction of MUC5AC gene expression by transfection with gob-5 or hCLCA1 genes in NCI-H292 cells. Total RNA was extracted from transfected cells 3 days after transfection, and reverse transcription (RT)-PCR amplification was performed for hCLCA1, gob-5, and MUC5AC gene detection. Amplification of the GAPDH gene was used as a control for RNA loading. cDNA reactions in which the RT was omitted (− lanes) were used as negative controls for each condition. These data are representative of three independent experiments.