Table 2.
Plasmids used in this study
| Name | Genotype | Source or reference |
|---|---|---|
| pAK6 | pADNX carrying pADH1-NUbiquitin-VMA13 | This study |
| pCT44 | pADNX carrying pADH1-NUbiquitin-EXO70 | This study |
| pKA86 | pRS316 carrying pGAL1-STE20-3HA | 57 |
| pKK19 | pRS316 carrying myc-VMA13 | 41 |
| pTH197 | pRS313 carrying pMET25-STE20-CUbiquitin-RURA3 | 57 |
| pTH263 | pRS425 carrying STE20 | This study |
| pTH380 | pRS425 carrying STE20-3HA | This study |
| pTH381 | pRS425 carrying STE20K649A-3HA | This study |
| pTH383 | pADNX carrying pADH1-NUbiquitin-VMA2 | This study |
| pTH385 | pRS313 carrying pMET25-STE14-CUbiquitin-RURA3 | This study |
| pTH386 | pADNX carrying pADH1-NUbiquitin-VMA3 | This study |
| MBP-Vma13 | pMALpAse carrying VMA13 | 7 |