Table 2.
Antifungal susceptibility testing for the C. albicans sam37ΔΔ mutant
| Antifungal drugb | MIC (μg/ml) |
MFC (μg/ml) |
||||||
|---|---|---|---|---|---|---|---|---|
| WT strain | sam37ΔΔ strain | sam37ΔΔ+SAM37 strain | Fold changea | WT | sam37ΔΔ strain | sam37ΔΔ+SAM37 strain | Fold changea | |
| Caspofungin | 0.25 | 0.25 | 0.25 | 1 | 0.5 | 0.25 | 0.5 | 2 |
| Congo red | 2 | 0.25 | 2 | 8 | 32 | 4 | 32 | 8 |
| Calcofluor white | 128 | 16 | 128 | 8 | >128 | 32 | >128 | >4 |
| Amphotericin B | 0.25 | 0.25 | 0.25 | 1 | 0.5 | 1 | 0.5 | 2 |
| Nystatin | 4 | 2 | 4 | 2 | 4 | 4 | 8 | 1 |
| Fluconazole | 0.5 | 0.25 | 0.5 | 2 | >64 | >64 | >64 | NA |
| Caspofungin (YPD) | 0.008 | 0.001 | 0.008 | 8 | 0.015 | 0.004 | 0.015 | 4 |
| Caspofungin (SC) | 0.25 | 0.06 | 0.25 | 4 | >8 | 1 | >8 | >8 |
a
Fold change: WT/sam37ΔΔ strain.Boldface indicates a significant change. NA, not available.
b
Unless otherwise stated, measurements were done using RPMI medium according to CLSI guidelines. SC, synthetic complete medium.