Fig 11.

LMBD2B has a role during development. (A) Cells were plated for development on nitrocellulose filters. At the indicated times (12, 16, and 20 h), images were taken using Metamorph software and a Dage-MTI video camera mounted on an Olympus dissecting microscope. The scale bar represents 1,000 μm. (B) Indirect immunofluorescence of LMBD2B-mRFP localization in whole mounts imaged on a Leica SP5 confocal microscope. Optical Z-axis slices through the developing structure are shown, with red signal indicating LMBD2B locations; shown are immunofluorescent images and overlays with transmitted images. The developing structure is a finger (∼16 h development).