Fig 4.

LMBD2B is localized in punctate regions on the cell periphery but clusters with prolonged substrate contact. Growing cells were placed on a coverslip for 15 min (A and B) or >2 h (C and D), fixed, and stained. Images of LMBD2B-mRFP indirect immunofluorescence are shown. Overlays of fluorescence over transmitted (trans) images are also shown. LMBD2B fluorescence is indicated by the red signal. (A and C) Z-axis slices through a cell (blue signal indicates nuclei stained with DAPI). (B and D) Side views of 3D-reconstructed cells; the white side bars indicate cell height.