Fig 7.

Vegetative LMBD2B-null cells show an elongated shape. Growing cells were placed on coverslips and allowed to sit overnight. The cells were stained with Alexa Fluor 488-phalloidin to label F-actin. (A) Images of the different cell types (an overlay of 3D flattened fluorescence and transmitted-light images). Polymerized actin is green. (B) Comparison of the roundness of WT and lmbd2B⁻ cells. ImageJ was used to calculate the roundness of the cells as follows; roundness = 4 × area/(π × major axis²). A perfect circle would equal a roundness of 1.0. lmbd2B⁻ cells were significantly less round (or more elongated) than WT cells. n = 25 cells from at least 3 different phalloidin stainings.