Table 1.
Bacterial strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Genotype or propertiesa | Reference |
|---|---|---|
| S. maltophilia | ||
| KJ | Wild type; a clinical isolate from Taiwan | 9 |
| KJΔZ | S. maltophilia KJ nagZ deletion mutant; ΔnagZ | This study |
| KJΔL1 | S. maltophilia KJ L1 isogenic mutant; L1::xylE | 9 |
| KJΔL1ΔZ | S. maltophilia KJ L1 and nagZ deletion mutant; L1::xylE ΔnagZ | This study |
| KJΔL2 | S. maltophilia KJ L2 isogenic mutant; L2::xylE | 9 |
| KJΔL2ΔZ | S. maltophilia KJ L2 and nagZ deletion mutant; L2::xylE ΔnagZ | This study |
| KJΔDI | S. maltophilia KJ ampDI deletion mutant; ΔampDI | 26 |
| KJΔDIΔZ | S. maltophilia KJ ampDI and nagZ deletion mutant; ΔAmpDI ΔnagZ | This study |
| KJΔR | S. maltophilia KJ ampR deletion mutant; ΔampR | 17 |
| KJΔNG | S. maltophilia KJ ampN and ampG deletion mutant; ΔampN ΔampG | 17 |
| KJΔmrcA | S. maltophilia KJ mrcA deletion mutant; ΔmrcA | 17 |
| KJΔmrcAΔZ | S. maltophilia KJ mrcA and nagZ deletion mutant; ΔmrcA ΔnagZ | This study |
| KJΔDIΔmrcA | S. maltophilia KJ ampDI and mrcA deletion mutant; ΔampDI ΔmrcA | 17 |
| KJΔDIΔmrcAΔZ | S. maltophilia KJ ampDI, mrcA, and nagZ deletion mutant; ΔAmpDI ΔmrcA ΔnagZ | This study |
| Escherichia coli | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Invitrogen |
| S17-1 | λ pir+ mating strain | 26 |
| Plasmids | ||
| pEX18Tc | sacB oriT Tcr | 7 |
| pRK415 | Mobilizable broad-host-range plasmid cloning vector, RK2 origin; Tcr | 14 |
| pX1918GT | Plasmid containing the xylE-gentamicin resistance gene cassette; Ampr Gmr | 23 |
| pΔZ | pEX18Tc with an internal-deletion nagZ gene; Tcr | This study |
| pRK-Z | pRK415 with a complete nagZ gene; Tcr | This study |
| pRK188ZxylE | pRK415 with a 188-bp DNA fragment upstream from the nagZ start codon and a nagZ::xylE transcriptional fusion | This study |
| pRKDI | pRK415 with a complete ampDI gene; Tcr | 26 |
| Primers | ||
| NagZ5-F | 5′-CTGCGCAAGCTTGCCGCCGAAC-3′ | This study |
| NagZ5-R | 5′-TTGCATGCCACCACCCCGGCCAC-3′ | This study |
| NagZ3-F | 5′-CACCGCATGCTGGTCGATGAAG-3′ | This study |
| NagZ3-R | 5′-CATGATCTAGAGGAACAGCGGCAC-3′ | This study |
| NagZP-F | 5′-CTGCGCAAGCTTGCCGCCGAAC-3′ | This study |
| NagZP-R | 5′-TTGCATGCCACCACCCCGGCCAC-3′ | This study |
a
Added restriction enzyme recognition sites in primers are underlined.