Fig 1.
Schematic representation of two novel class I integrons containing blaIMP-18. Two arrangements of the class 1 integron containing the blaIMP-18 gene were identified by a PCR mapping strategy described by Sánchez-Martínez et al. (14). In both class I integrons, the blaIMP-18 gene is located in the first position next to the 5′ conserved region. (A) In706, the disrupted aacA43Δ gene cassette (GC) was identified at the second position. blaOXA-2 and gcuD of a hypothetical orfD gene of unknown function were identified in the third and fourth positions, respectively. (B) The diagram of In707 shows the blaIMP-18 gene followed by aadA1b and blaOXA-224. Open reading frames (ORFs) attl1 and attC are indicated by arrows with different gray colors. Scale: 1 cm = 200 bp. Illustration courtesy of the INTEGRALL database (http://integrall.bio.ua.pt/).