Fig 3.

Defects in cell wall integrity in some of the ergosterol biosynthesis inhibitor-sensitive mutants. (A) The Δgpa2 mutants, a representative example of the ergosterol biosynthesis inhibitor-sensitive mutants, were hypersensitive to micafungin, a (1,3)-β-d-glucan synthase inhibitor. Cells were spotted onto plates containing YES or YES plus 2.0 μg/ml micafungin and incubated at 27°C for 4 days. (B) High osmolarity suppressed the ergosterol biosynthesis inhibitor-sensitive phenotype of the Δgpa2 mutants. Wild-type cells and Δgpa2 mutant cells were spotted onto each plate as indicated. (C) Cell wall digestion of the deletion mutants and wild-type cells by β-glucanase. Cells exponentially growing in YES medium were harvested, incubated with β-glucanase at 27°C, and subjected to vigorous shaking. Cell lysis was monitored by measurements of the optical density at 660 nm. The data shown are representative of multiple experiments.