Table 1.
S. pombe genes identified in the screen for clotrimazole- and terbinafine-sensitive genesf
| Category and systematic gene name | Common gene name | Gene descriptiona | Spot assay result with: |
MIC (μg/ml)e |
||
|---|---|---|---|---|---|---|
| Clot | Terb | Clot | Terb | |||
| Wild type | 2.0 | 0.25 | ||||
| Ergosterol synthesis | ||||||
| SPAC20G4.07c | sts1 | C-24(28) sterol reductase Sts1 | +++ | +++ | 0.06 | 0.06 |
| SPBC36.06c | spo9 | Farnesyl pyrophosphate synthetase | ++ | +++ | 1.0 | 0.03 |
| SPBC1271.12 | kes1 | Oxysterol binding protein | +++ | +++ | 0.25 | 0.12 |
| SPAC25B8.01 | dap1 | Cytochrome P450 regulator Dap1 | ++ | − | 1.0 | 0.25 |
| Membrane trafficking | ||||||
| SPAC144.06 | apl5 | AP-3 adaptor complex subunit Apl5 | +++ | + | 0.25 | 0.12 |
| SPAC23H3.06 | apl6 | AP-3 adaptor complex subunit Apl6 | +++ | + | 0.25 | 0.12 |
| SPAC30D11.05 | aps3 | AP-3 adaptor complex subunit Aps3 | +++ | + | 0.5 | 0.25 |
| SPBC651.11c | apm3 | AP-3 adaptor complex subunit Apm3 | + | + | 1.0 | 0.25 |
| SPBP16F5.07 | apm1 | AP-1 adaptor complex subunit Apm1 | +++ | + | 0.06 | 0.12 |
| SPAC767.01c | vps1 | Dynamin family protein Vps1 | +++ | + | 0.06 | 0.12 |
| SPAC2G11.03c | vps45 | Vacuolar sorting protein Vps45 | +++ | ++ | 0.12 | 0.015 |
| SPAC1142.07c | vps32 | Vacuolar sorting protein Vps32 | ++ | + | 0.12 | 0.12 |
| SPBC27B12.08 | sip1 | Pof6 interaction protein Sip1 | ++ | + | 0.12 | 0.25 |
| SPAC31A2.13c | sft1c | SNARE Sft1 | +++ | + | 0.12 | 0.06 |
| SPAC1527.02 | sft2 | Golgi transport protein Sft2 | + | ++ | 0.12 | 0.03 |
| SPAC4G8.10 | gos1 | SNARE Gos1 | − | + | 0.5 | 0.12 |
| SPAC869.11 | cat1 | Cationic amino acid transporter Cat1 | +++ | ++ | 1.0 | 0.12 |
| SPCC1795.03 | gms1 | UDP-galactose transporter Gms1 | ++ | + | 0.25 | 0.06 |
| SPAC23C11.14 | zhf1 | Zinc ion transporter Zhf1 | +++ | + | 0.25 | 0.06 |
| SPBC25H2.16c | gga1b | Adaptin | ++ | +++ | 0.25 | 0.03 |
| SPCC794.11c | ent3b | ENTH/VHS domain protein Ent3 | ++ | + | 0.25 | 0.06 |
| SPCC794.03 | uga4b | Amino acid permease | +++ | + | 0.25 | 0.12 |
| SPBC725.10 | NA | TspO homolog | +++ | + | 0.06 | 0.06 |
| Histone acetylation and deacetylation | ||||||
| SPCC24B10.08c | ada2 | SAGA complex subunit Ada2 | +++ | + | 0.06 | 0.12 |
| SPBC28F2.10c | ada3c | SAGA complex subunit Ngg1 | +++ | − | 0.25 | 0.25 |
| SPBC14C8.17c | spt8c | SAGA complex subunit Spt8 | ++ | + | 0.25 | 0.12 |
| SPBC146.09c | lsd1 | Histone demethylase SWIRM1 | ++ | ++ | 0.25 | 0.06 |
| SPAC16C9.05 | cph1 | Clr6 histone deacetylase-associated PHD protein 1 (Cph1) | ++ | ++ | 0.12 | 0.12 |
| SPCC126.13c | NA | Histone deacetylase complex subunit | − | + | 0.25 | 0.06 |
| Ubiquitination | ||||||
| SPAC11G7.02 | pub1 | Ubiquitin-protein ligase E3 | +++ | + | 0.06 | 0.03 |
| SPBC21D10.09c | rkr1b | Ubiquitin-protein ligase E3 | +++ | + | 0.12 | 0.03 |
| SPBC14F5.10c | NA | Ubiquitin-protein ligase E3 | +++ | ++ | 0.5 | 0.06 |
| SPAC13A11.04c | ubp8 | Ubiquitin C-terminal hydrolase | ++ | + | 0.12 | 0.12 |
| SPCC1223.01 | NA | Ubiquitin-protein ligase E3 | +++ | + | 0.12 | 0.06 |
| SPAC17H9.19c | cdt2 | WD repeat protein Cdt2 | ++ | ++ | 0.25 | 0.03 |
| SPAC1687.05 | pli1 | SUMO E3 ligase Pli1 | + | + | 0.5 | 0.12 |
| SPCC970.10c | brl2 | Ubiquitin-protein ligase E3 | ++ | ++ | 0.03 | 0.03 |
| SPAC17H9.10c | ddb1 | Damaged DNA binding protein | +++ | ++ | 0.25 | 0.03 |
| Signal transduction | ||||||
| SPCC1753.02c | git3c | G-protein-coupled receptor Git3 | +++ | + | 0.12 | 0.06 |
| SPAC23H3.13c | gpa2 | Heterotrimeric G protein α-2 subunit | +++ | +++ | 0.12 | 0.06 |
| SPBC32H8.07 | git5 | Heterotrimeric G protein b subunit | +++ | + | 0.25 | 0.06 |
| SPBC215.04 | git11 | Heterotrimeric G protein γ subunit | ++ | + | 0.25 | 0.06 |
| SPBC21C3.20c | git1 | C2 domain protein Git1 | +++ | − | 0.12 | 0.06 |
| SPCC162.10 | ppk33 | Serine/threonine protein kinase | ++ | ++ | 0.25 | 0.06 |
| SPBC106.10 | pka1 | cAMP-dependent protein kinase catalytic subunit Pka1 | +++ | ++ | 0.12 | 0.03 |
| SPAC6F6.01 | cch1 | Calcium channel Cch1 | ++ | ++ | 0.06 | 0.03 |
| SPAC16.01 | rho2 | Rho family GTPase Rho2 | ++ | ++ | 0.25 | 0.06 |
| SPBC15D4.15 | pho2 | 4-Nitrophenylphosphatase | +++ | + | 1.0 | 0.12 |
| SPAC4F10.04 | rrd1b | Protein phosphatase type 2A, intrinsic regulator | ++ | + | 0.12 | 0.03 |
| SPCC757.09c | rnc1 | RNA binding protein that suppresses calcineurin deletion Rnc1 | + | − | 0.25 | 0.25 |
| SPCC297.05c | NA | Diacylglycerol binding protein | +++ | ++ | 0.25 | 0.06 |
| Ribosome biogenesis and assembly | ||||||
| SPAC3A12.10 | rpl2001 | 60S ribosomal protein L20a | +++ | ++ | 0.12 | 0.03 |
| SPAC26A3.07c | rpl1101 | 60S ribosomal protein L11 | ++ | + | 0.5 | 0.12 |
| SPAC3H5.10 | rpl3202 | 60S ribosomal protein L32 | ++ | + | 0.25 | 0.12 |
| SPAC1556.05c | cgr1c | Ribosome biogenesis CGR1 family | ++ | + | 0.5 | 0.12 |
| SPBC16C6.03c | rsa1b | Ribosome assembly protein | + | + | 2.0 | 0.12 |
| SPAC6F6.03c | nog2b | Ribosome export GTPase | +++ | ++ | 0.25 | 0.12 |
| Regulation of transcription and translation | ||||||
| SPBC21B10.13c | yox1 | MBF complex negative regulatory component Yox1 | +++ | + | 0.25 | 0.06 |
| SPBC2F12.11c | rep2 | Transcriptional activator, MBF subunit | + | ++ | 0.25 | 0.03 |
| SPBC19G7.16 | iws1 | Transcription elongation factor complex subunit Iws1 | ++ | ++ | 0.5 | 0.12 |
| SPBC31F10.09c | nut2 | Mediator complex subunit Med10 | ++ | ++ | 0.5 | 0.12 |
| Cellular morphogenesis, cell wall organization, and biogenesis | ||||||
| SPBC947.04d | NA | Cell surface glycoprotein, DIPSY family | +++ | ++ | 0.12 | 0.06 |
| SPAC1F8.06 | fta5c | Cell surface glycoprotein | ++ | + | 0.25 | 0.25 |
| SPCC63.02c | aah3 | Alpha-amylase homolog Aah3 | + | − | 0.5 | 0.25 |
| SPCP1E11.04c | pal1c | Membrane-associated protein | ++ | + | 0.12 | 0.015 |
| SPAC688.11 | end4 | Huntingtin-interacting protein homolog | +++ | + | 0.03 | 0.06 |
| Mitochondrial function | ||||||
| SPAC1071.11 | NA | NADH-dependent flavin oxidoreductase | ++ | ++ | 0.03 | 0.015 |
| SPAC1556.02c | sdh1 | Succinate dehydrogenase Sdh1 | +++ | + | 0.25 | 0.12 |
| SPAC4G8.08 | mrs3b | Mitochondrial iron ion transporter | − | + | 0.5 | 0.12 |
| Amino acid metabolism | ||||||
| SPAC3A12.17c | cys12 | Cysteine synthase Cys12 | +++ | + | 0.12 | 0.06 |
| SPBC3B8.03 | lys9b | Saccharopine dehydrogenase | +++ | + | 0.12 | 0.12 |
| SPAC3H1.07 | aru1 | Arginase Aru1 | + | − | 0.5 | 0.25 |
| SPAC24H6.10c | aro3b | Phospho-2-dehydro-3-deoxyheptonate aldolase | + | − | 0.25 | 0.25 |
| Nucleic acid metabolism | ||||||
| SPAC644.14c | rhp51 | Recombinase Rhp51 | +++ | + | 0.12 | 0.03 |
| SPBC29A3.14c | trt1 | Telomerase reverse transcriptase 1 protein | − | + | 0.5 | 0.12 |
| SPAPB1E7.02c | mcl1 | DNA polymerase alpha accessory factor | + | + | 0.25 | 0.06 |
| SPCC736.09c | NA | TRAX | + | + | 1.0 | 0.25 |
| SPAC30.02c | kti12b | Elongator complex-associated protein | +++ | − | 0.03 | 0.12 |
| SPBC36.07 | iki3 | Elongator subunit Iki3 | +++ | + | 0.25 | 0.06 |
| SPCC31H12.08c | ccr4 | CCR4-Not complex subunit Ccr4 | ++ | + | 0.25 | 0.12 |
| SPAC9G1.12 | cpd1 | tRNA (m1A) methyltransferase complex subunit Cpd1 | +++ | ++ | 0.25 | 0.03 |
| SPBC25D12.06c | NA | RNA helicase | +++ | + | 0.25 | 0.06 |
| Lipid metabolism | ||||||
| SPBC31F10.02 | NA | Acyl-CoA thioesterase | +++ | + | 0.12 | 0.06 |
| SPCC1235.15 | dga1 | Diacylglycerol O-acyltransferase | +++ | + | 0.12 | 0.06 |
| SPAC1786.01c | tgl4b | Triacylglycerol lipase | +++ | + | 0.5 | 0.06 |
| Meiosis | ||||||
| SPBC359.06 | mug14 | Adducin | ++ | + | 0.25 | 0.12 |
| SPAC3A11.02 | cps3c | Zinc finger protein Cps3 | +++ | + | 0.12 | 0.03 |
| SPBC577.12 | mug71 | Endoribonuclease | + | + | 0.12 | 0.12 |
| SPCC338.08 | ctp1 | CtIP-related endonuclease | + | + | 0.25 | 0.06 |
| SPAC110.02 | pds5 | Cohesin-associated protein Pds5 | + | + | 0.25 | 0.12 |
| SPAC15A10.03c | rhp54 | Rad54 homolog Rhp54 | ++ | + | 0.25 | 0.06 |
| Other functions | ||||||
| SPAC1D4.05c | erd1b | Erd1 homolog | ++ | + | 0.12 | 0.03 |
| SPBC1709.14 | png1b | Peptide N-glycanase | +++ | + | 0.12 | 0.03 |
| SPBC365.14c | uge1 | UDP-glucose 4-epimerase | + | + | 0.5 | 0.06 |
| SPAC19B12.08 | atg4 | Atg8 deconjugator Atg4 | + | + | 0.5 | 0.12 |
| SPAC823.16c | mug179 | WD repeat protein involved in autophagy Atg18b | ++ | ++ | 0.5 | 0.06 |
| SPCC794.10 | ugp1b | UTP-glucose-1-phosphate uridylyltransferase | + | + | 0.25 | 0.06 |
| SPBP35G2.14 | puf1b,c | RNA binding protein | + | + | 0.25 | 0.12 |
| SPAC12G12.03 | cip2 | RNA binding protein Cip2 | + | ++ | 0.015 | 0.015 |
| Unknown functions | ||||||
| SPAC19A8.11c | irc6b | Recombination protein Irc6 | +++ | − | 0.06 | 0.25 |
| SPCC1020.07 | NA | Haloacid dehalogenase-like hydrolase | +++ | + | 0.25 | 0.06 |
| SPBC2A9.11c | thp3b | Nuclear export factor | +++ | + | 0.12 | 0.06 |
| SPAC56E4.07c | NA | N-Acetyltransferase | +++ | + | 0.12 | 0.06 |
| SPAC57A10.08cc | NA | Carboxylic ester hydrolase activity | +++ | ++ | 0.06 | 0.03 |
| SPBC660.05 | wwm1b,c | Conserved fungal protein | +++ | + | 0.12 | 0.06 |
| SPAC22F8.03cd | NA | Sequence orphan | +++ | ++ | 0.12 | 0.06 |
| SPCC622.14 | gcs1b | GTPase-activating protein | +++ | ++ | 0.12 | 0.03 |
| SPCC1739.01 | lee1b,c | zf-CCCH-type zinc finger protein | +++ | + | 0.12 | 0.06 |
| SPAC1805.14d | NA | Sequence orphan | + | + | 0.12 | 0.12 |
a
Gene description as indicated in the Sanger Center S. pombe database. WD, tryptophan-aspartic acid; MBF, MluI cell cycle box binding complex; CtIP, CtBP (carboxy-terminal binding protein) interacting protein; TRAX, translin-associated protein X homolog.
b
The common name is taken from the orthology of S. cerevisiae.
c
The gene is conserved in fungi only.
d
The gene is identified in S. pombe only.
e
Values obtained by using the MIC-2 (50% growth inhibition) endpoint.
f
+++, severely sensitive; ++, moderately sensitive; +, mildly sensitive; −, not sensitive; Clot, clotrimazole; Terb, terbinafine; NA, the common gene name is not applicable.